วัสดุตรึงจุลินทรีย์จากของเหลือทิ้งทางการเกษตร= Immobilized Material form Agriculture Residue/ Rachain Visutthipat [et al.] (CONFIDENTIAL)
โดย: Rachain Visutthipat
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ชื่อชุด: Res. Proj. no. 58-11, Sub Proj. no. 2; no.1 (Final report) (CONFIDENTIAL)ข้อมูลการพิมพ์: Pathumthani : Thailand Institute of Scientific and Technological Research, 2021 รายละเอียดตัวเล่ม: 74 p. 30 cm.ชื่อเรื่องอื่นๆ: โครงการวิจัยที่ ภ.58-11 วัสดุตรึงจุลินทรีย์จากของเหลือทิ้งทางการเกษตรหัวเรื่อง: วัสดุตรึงจุลินทรีย์สาระสังเขป: The results of a survey on the amount of glyphosate used by farmers in Chiang Rai, Phayao, Nan, Lopburi and Phetchabun showed that 92 percent of the farmers had used glyphosate with 70 percent of the surveyed repeatedly exceeding the recommended proportion in each use over the period of 60-80 days. In the examination, bacteria with the potential of digesting were isolated from the soil by enrichment culture technique and cultured on Tryptic Soy Agar (TSA). At the end of the examination, three different isolates of effective biodegradation namely GLY68, GLY67 and GLY79 had been identified. In the experiment, all three isolates were able to grow in glyphosate concentration of 20 ppm. When tested at the pH level of 4-10, and with the salinity levels up to 5 percent, all three bacteria were still able to grow even in anaerobic conditions. However, when the glyphosate concentration was increased to200 ppm, the isolate GLY68 showed the maximum biodegradation of glyphosate degrading91.65 percent of the glyphosate within the 5 days. The development of immobilized material from agriculture residue, waste wood, bamboo, coconut shell and the core corn were burned at 550 ° C for 3 hours before a group of bacteria was inserted into each of the four immobilized materials. The result showed that immobilized material from coconut shells always release the maximum number of microorganisms. Regarding the survival test of the microorganisms on the 4 immobilized materials, it was found that the material that gave the longest survival period of 180 days was coconut shell charcoal, followed by corn-core charcoal with the survival rate of 2.7 × 106 and 2.6 × 106, respectively. Regarding glyphosate biodegradation test at the pot level, the microbial from corn-core charcoal was able to degrade glyphosate with 49.45, 20.48, 5.62 and 2.08 percent of glyphosate left over the period of 5, 10, 15 and 30 days, respectively. In comparison to glyphosate biodegradation test at a farming scale, the rate of biodegradation increased with days from 37.5, 66.67, 87.5, 91.67, 96.5 and 98.33 percent of glyphosate over the period of 0, 5, 10, 15, 20, 25 and 30 days, respectively
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